CellCount.

Reproducible cell counts, through your own microscope. Trypan blue viability in seconds, whether the image comes from a chamber under the microscope or from the counter the lab already owns, with every count returned on its image, with the metrics behind it.

Built on the hardware the lab already owns.

A camera on the scope

A plug-and-play camera on the microscope port, counting straight into CellCount on the laptop at the bench. The recommended setup.

A phone at the eyepiece

Hold a modern phone to the eyepiece and count from the photo. No adapter, no hardware at all.

Images you already have

Drop in files from any microscope, or a whole export folder from an automated counter, and the same algorithm runs on them as on a chamber under a microscope.

An inverted microscope with a Scope Cam on its camera port, and beside it a touchscreen PC showing CellCount with the counted grid and the concentration in cells per mL
A Scope Cam on the microscope and CellCount on a touchscreen PC beside it, at ShareLab.

One upload, the whole answer, and the record.

The count, on the image

Every detected cell marked on the lab’s own frame, the grid it measured, the concentration in cells per mL from that grid and the dilution, the viability, and a cell-size histogram with the gate the lab set.

One protocol for the whole lab

Dilution, which squares, sensitivity and size gate set once and shared by the team, so counts agree between people, instruments and days.

A record someone signed

Each sample is dated, accepted by a named person and sealed with a hash. It leaves as a results document, or as an export bundle of the images with their overlays and results, for the lab’s own archive.

Tested on frames it never saw.

Measured against the instrument labs already trust: sixteen images straight from a leading automated cell counter, which CellCount had never seen. The method, the plots and the sources are on the validation page.

0.91Lin’s concordance on cell concentration with a leading automated cell counter, across sixteen images
±0.29million cells/mL, the 95% limits of agreement, with a mean difference of 0.002
95%the same live-or-dead call on the 6,765 cells both readers found
0images from that instrument in CellCount’s training data

Free to read and use, no account.

Guides and a calculator for counting cells by hand, on cellcount.ai.

Hemocytometer calculator

The cell count formula with worked examples, and a calculator for cells per mL, viability and the volume to seed.

How to count cells with a hemocytometer

The manual method step by step: trypan blue, loading the chamber, which squares, the arithmetic and a worked example.

Count cells from a phone photo

What the photo needs when a phone is held to the eyepiece of the microscope the lab already has.

Cell counting terms, defined

Hemocytometer, trypan blue exclusion, viability, dilution factor and more, each with its published source.